TamiFlu Resistant Australian Sequences Demonstrate Recombination with Human and Avian H5N1
Posted by labvirus on June 13, 2011
1. Hurt AC, Lee RT, Leang SK, Cui L, Deng YM, Phuah SP, Caldwell N, Freeman K, Komadina N, Smith D, Speers D, Kelso A, Lin RT, Maurer-Stroh S, Barr IG. Increased detection in Australia and Singapore of a novel influenza A(H1N1)2009 variant with reduced oseltamivir and zanamivir sensitivity due to a S247N neuraminidase mutation. Euro Surveill. 2011;16(23):pii=19884.
Available online: //www.eurosurveillance.org/ViewArticle.aspx?ArticleId=19884
Date of submission: 26 May 2011
“A novel influenza A(H1N1)2009 variant with mildly reduced oseltamivir and zanamivir sensitivity has been detected in more than 10% of community specimens in Singapore and more than 30% of samples from northern Australia during the early months of 2011.”1
On 2011-06-02, the WHO Collaborating Centre for Reference and Research on Influenza published 10 HA and 12 NA pH1N1 sequence segments at GISAID in support of their paper 1 on the MultiDrug Resistance moderated by NA 247N. These sequences were sampled from patients in Australia and Brunei from January to March 2011. In addition to the discussed NA 247N, all 12 Neuraminidase segments display multiple polymorphism recombination at the 5’ end with a zoonotic co-infection from a strain matching Human and Avian H5N1 cases as well as H4, H6, H9N2, H10, H11, H12 and H13 sequences. The Brunei sequence exhibits potential recombination to the same zoonotic reservoirs with additional NA nucleotide homology at aa40, aa46, aa95, 157T, aa353, 369K, aa377 and aa426.
These sequences are deeply avian in nature with additional homology to various zoonotic reservoirs. Though the primary subclade carrying this drug resistance marker is young, the basic backbone has risen to triple digits rather quickly. The HA and the NA segments in this deposit represent hypermorphic outcomes signaling a change in viral behaviour on SubClade 5 (208K_219V) in this region of the world during early 2011. Notice must be made that the NA 247N has crossed onto the HA 188T SubClade as well. Existence of the newly recognised TamiFlu resistance marker across multiple human pandemic subclades indicates potential for spread.
As we have previously discussed concerning genetic markers for drug resistance, GeneWurx expects that standing genetic revisions on the pH1N1 subclades will additionally be laboratory validated to drug resistance.
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